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Image Search Results
Journal: Chemical & Biomedical Imaging
Article Title: Nonemissive Iridium(III) Solvent Complex as a Self-Reporting Photosensitizer for Monitoring Phototherapeutic Efficacy in a “Signal on” Mode
doi: 10.1021/cbmi.4c00042
Figure Lengend Snippet: Evaluation for hallmarks of ICD in HeLa cells upon treatment with Ir-DMSO in the dark or upon white light irradiation. Immunofluorescence confocal laser scanning microscopy stained with (A) Alexa Fluor 488 conjugate calreticulin rabbit mAb and (C) Alexa Fluor 555 conjugate antirabbit IgG (H+L) and HMGB1 antibody. Analysis of CRT (B) and HMGB1 (D) levels in HeLa cells from (A) and (B). (E) Release of adenosine triphosphate into the cell culture supernatant. Probabilities * p < 0.05, ** p < 0.01, *** P < 0.001 are marked in the figure and 0.05 was chosen as the significance level.
Article Snippet: Alexa Fluor 555 conjugate antirabbit IgG (H+L), alexa Fluor 488 conjugate CRT rabbit mAb, HMGB1 antibody and
Techniques: Irradiation, Immunofluorescence, Confocal Laser Scanning Microscopy, Staining, Cell Culture